Active Recombinant Human FSH α/β protein

• Cat.No.: FSH-262H
• Product Overview: Recombinant Human FSH α/β, FSH α(Ala25-Ser116)) and FSH β(Cys21-Glu129), was expressed in CHO.

Specification

• Description: Follicle-stimulating hormone (FSH) is a hormone found in humans and other animals. It is synthesized and secreted by gonadotrophs of the anterior pituitary gland. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits. The alpha subunits contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHβ), which confers its specific biologic action and is responsible for interaction with the FSH receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3–4 hours. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body.
• Source: CHO
• Species: Human
• Form: Prepared in PBS (1 mg/mL), Filtered (0.22 μm) & lyophilized.
• Bio-activity: ED50 = 0.1 to 0.4 ng/ml in human FSH receptor transfected HEK 293 cells.
• Molecular Mass: Predicted: 10 kDa (FSH α) & 12 kDa (FSH β), ~25.0 KDa by SDS-PAGE.
• Endotoxin: <1.0 EU per 1μg of protein (by Limulus Amoebocyte Lysate Test)
• Purity: 0.95
• Applications: WB, ELISA, Cell culture
• Storage: Store lyophilized and reconstituted proteins at -20 centigrade for Long Term and at 4 centigrade for < 2 weeks. Avoid repeated freezing/thawing cycles.
• Reconstitution: Reconstitute at 100 ug/ml in sterile water/PBS containing at least 0.1% human or bovine serum albumin

Gene Information

• Gene Name: FSHB follicle stimulating hormone, beta polypeptide [ Homo sapiens ]
• Official Symbol: FSHB
• Synonyms: FSHB; follicle stimulating hormone, beta polypeptide; follitropin subunit beta; follicle stimulating hormone beta subunit; follitropin; beta chain; FSH-B; FSH-beta; follitropin beta chain; follitropin, beta chain; follicle-stimulating hormone beta subunit
• Gene ID: 2488
• mRNA Refseq: NM_000510
• Protein Refseq: NP_000501
• MIM: 136530
• UniProt ID: P01225
• Chromosome Location: 11p13
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glycoprotein hormones, organism-specific biosystem; GnRH signaling pathway, organism-specific biosystem; GnRH signaling pathway, conserved biosystem
• Function: follicle-stimulating hormone activity; hormone activity; protein binding; protein heterodimerization activity