Active Recombinant Human FUT8 protein, His-tagged

• Cat.No.: FUT8-2518H
• Product Overview: Active Recombinant Human FUT8 protein(Q9BYC5-1) (Arg 68-Lys 575) was expressed in Insect Cells, fused with a polyhistidine tag at the carboxy-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 68-575 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly.
• Bio-activity: Measured by its ability to hydrolyze the donor substrate GDP fucose. The specific activity is >0.75 pmoles/min/μg.
• Molecular Mass: The recombinant human FUT8 consists of 518 amino acids and has a calculated molecular mass of 60 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies (2021)

Gene Information

• Gene Name: FUT8 fucosyltransferase 8 (alpha (1,6) fucosyltransferase) [ Homo sapiens ]
• Official Symbol: FUT8
• Synonyms: FUT8; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); alpha-(1,6)-fucosyltransferase; alpha1-6FucT; glycoprotein 6-alpha-L-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; MGC26465
• Gene ID: 2530
• mRNA Refseq: NM_004480
• Protein Refseq: NP_004471
• MIM: 602589
• UniProt ID: Q9BYC5