Active Recombinant Human GCGR protein, Fc-tagged

• Cat.No.: GCGR-1494H
• Product Overview: Recombinant Human GCGR protein(Ala26-Lys136), fused with Fc tag, was expressed in CHO .

Specification

• Source: CHO
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human GCGR Protein, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-GCGR Antibody，Human IgG4 with a linear range of 0.5-16 ng/mL.Immobilized Human GCGR Protein, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-GCGR Antibody，Human IgG2 with a linear range of 0.2-16 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 39.5 kDa. The protein migrates as 52-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala26-Lys136
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GCGR glucagon receptor [ Homo sapiens ]
• Official Symbol: GCGR
• Synonyms: GCGR; glucagon receptor; GGR; GL-R; FLJ97182; MGC138246
• Gene ID: 2642
• mRNA Refseq: NM_000160
• Protein Refseq: NP_000151
• MIM: 138033
• UniProt ID: P47871