Active Recombinant Human GCGR protein, His-Avi-tagged, Biotinylated

Species :	Human
Source :	HEK293
Tag :	Avi&His
Protein Length :	26-136 a.a.
Form :	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Bio-activity :	Biotinylated Human GCGR / Glucagon receptor, His,Avitag immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 48.5 μM as determined in a SPR assay (Biacore 8K).
Molecular Mass :	This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 16.6 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength :	Ala26-Lys136
Endotoxin :	Less than 1.0 EU per μg by the LAL method.
Purity :	>90% as determined by SDS-PAGE.
Storage :	For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Conjugation :	Biotin

Gene Name	GCGR glucagon receptor [ Homo sapiens ]
Official Symbol	GCGR
Synonyms	GCGR; glucagon receptor; GGR; GL-R; FLJ97182; MGC138246;
Gene ID	2642
mRNA Refseq	NM_000160
Protein Refseq	NP_000151
MIM	138033
UniProt ID	P47871