Active Recombinant Human GCGR protein, His-Avi-tagged, Biotinylated

• Cat.No.: GCGR-1495H
• Product Overview: Biotinylated Recombinant Human GCGR protein(Ala26-Lys136), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Biotinylated Human GCGR / Glucagon receptor, His,Avitag immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 48.5 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 16.6 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala26-Lys136
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GCGR glucagon receptor [ Homo sapiens ]
• Official Symbol: GCGR
• Synonyms: GCGR; glucagon receptor; GGR; GL-R; FLJ97182; MGC138246
• Gene ID: 2642
• mRNA Refseq: NM_000160
• Protein Refseq: NP_000151
• MIM: 138033
• UniProt ID: P47871