Active Recombinant Human GNLY protein, His-tagged

• Cat.No.: GNLY-4381H
• Product Overview: Recombinant Human GNLY(Met1-Leu145) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-145 a.a.
• Predicted N Terminal: Arg23
• Form: Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
• Bio-activity: Measured by its ability to induce RANTES secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is typically 0.3-1 µg/mL, in the presence of 10 µg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: Predicted Molecular Mass: 15.4 kDa;SDS-PAGE: 15 - 16 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: GNLY granulysin [ Homo sapiens ]
• Official Symbol: GNLY
• Synonyms: GNLY; granulysin; LAG2; D2S69E; LAG 2; NKG5; T lymphocyte activation gene 519; TLA519; lymphokine LAG-2; lymphocyte-activation gene 2; T-cell activation protein 519; T-lymphocyte activation gene 519; 519; LAG-2
• Gene ID: 10578
• mRNA Refseq: NM_006433
• Protein Refseq: NP_006424
• MIM: 188855
• UniProt ID: P22749
• Chromosome Location: 2p12-q11