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Active Recombinant Human GPC3 Protein (Gln 25 - His 559), Fc-tagged, FITC-Labeled
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Cat.No. : | GPC3-338H Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654). It is the FITC labeled form of Human Glypican 3, Fc Tag. Predicted N-terminus: Gln 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. The protein migrates as 43 kDa, 58 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 3-5. |
Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Anti-Human GPC3 MAb at 10 μg/mL (100 μL/well) can bind Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled with a linear range of 0.078-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested). |
Molecular Mass : | 87.4 kDa |
Protein length : | Gln 25 - His 559 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Figure : |
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Gene Name : | GPC3 glypican 3 [ Homo sapiens ] |
Official Symbol : | GPC3 |
Synonyms : | GPC3; glypican 3; SDYS; glypican-3; DGSX; OCI 5; SGB; SGBS; SGBS1; MXR7; OCI-5; GTR2-2; |
Gene ID : | 2719 |
mRNA Refseq : | NM_001164617 |
Protein Refseq : | NP_001158089 |
MIM : | 300037 |
UniProt ID : | P51654 |
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