GPC3-338H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654). It is the FITC labeled form of Human Glypican 3, Fc Tag. Predicted N-terminus: Gln 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. The protein migrates as 43 kDa, 58 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 3-5.
Source :
HEK293
Species :
Human
Tag :
Fc
Form :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity :
Immobilized Anti-Human GPC3 MAb at 10 μg/mL (100 μL/well) can bind Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled with a linear range of 0.078-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
Molecular Mass :
87.4 kDa
Protein length :
Gln 25 - His 559
Endotoxin :
Less than 1.0 EU per μg by the LAL method.
Purity :
>90% as determined by SDS-PAGE.
Notes :
Please avoid repeated freeze-thaw cycles.
Stability :
No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
Storage :
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.