Active Recombinant Human HS3ST4 Protein, His-tagged

• Cat.No.: HS3ST4-564H
• Product Overview: Recombinant Human HS3ST4 (Accession # NP_006031) Gly184-Lys456, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: Gly184-Lys456
• Predicted N Terminal: HHHHHHGGAV
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to heparan sulfate.The specific activity is >5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human HS3ST4, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 35-43 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: HS3ST4 heparan sulfate (glucosamine) 3-O-sulfotransferase 4 [ Homo sapiens ]
• Official Symbol: HS3ST4
• Synonyms: HS3ST4; heparan sulfate (glucosamine) 3-O-sulfotransferase 4; heparan sulfate glucosamine 3-O-sulfotransferase 4; 3OST4; heparan sulfate 3-O-sulfotransferase 4; heparan sulfate 3-O-sulfotransferase-4; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4; 30ST4; 3-OST-4; h3-OST-4
• Gene ID: 9951
• mRNA Refseq: NM_006040
• Protein Refseq: NP_006031
• MIM: 604059
• UniProt ID: Q9Y661
• Chromosome Location: 16p11.2