Active Recombinant Human ICOSLG protein, His-tagged

• Cat.No.: ICOSLG-3213H
• Product Overview: Active Recombinant Human ICOSLG protein(NP_056074.1)(Met 1-Ser 258) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-258 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.
• Molecular Mass: The secreted recombinant human ICOSLG consists of 251 amino acids and has a calculated molecular mass of 28 kDa. The apparent molecular mass of the protein is approximately 50-60 kDa in SDS-PAGE under reducing conditions as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ICOSLG inducible T-cell co-stimulator ligand [ Homo sapiens ]
• Official Symbol: ICOSLG
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; ICOSL; ICOS ligand; B7 homolog 2; B7 homologue 2; B7 H2; B7 related protein 1; B7H2; B7RP 1; B7RP1; CD275; GL50; ICOS L; KIAA0653; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand; B7-H2; LICOS; B7RP-1; ICOS-L
• Gene ID: 23308
• mRNA Refseq: NM_015259
• Protein Refseq: NP_056074
• MIM: 605717
• UniProt ID: O75144