Active Recombinant Human IL10 protein, His-tagged

• Cat.No.: IL10-372H
• Product Overview: Active Recombinant Human IL10 protein(NP_000563.1) (Ser 19-Asn 178) was expressed in HEK293, with a N-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-178 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Immobilized human IL10-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.31-0.71 μg/ml. 2. Measured in a cell liferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 5-20 ng/mL.
• Molecular Mass: The recombinant human IL10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000563.1
• Unit ID: P22301
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P22301

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; interleukin-10; CSIF; cytokine synthesis inhibitory factor; IL 10; IL10A; T cell growth inhibitory factor; TGIF; T-cell growth inhibitory factor; GVHDS; IL-10; MGC126450; MGC126451
• Gene ID: 3586
• mRNA Refseq: NM_000572
• protein Refseq: NP_000563
• MIM: 124092