Active Recombinant Human IL10RB protein, His&hFc-tagged

• Cat.No.: IL10RB-176H
• Product Overview: Active Recombinant Human IL10RB protein(Met 1-Ser 220)(NP_000619.3) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-220 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB-Fc with a linear ranger of 3.125-25 μg/ml.
• Molecular Mass: The recombinant human IL10Rβ/Fc is a disulfide-linked homodimer. The reduced monomer consists of 449 amino acids and has a predicted molecular mass of 51.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL10Rβ/Fc monomer is apximately 75-85 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000619.3
• Unit ID: Q08334
• Unit ID-Weblink: http://www.uniprot.org/uniprot/Q08334

Gene Information

• Gene Name: IL10RB interleukin 10 receptor, beta [ Homo sapiens ]
• Official Symbol: IL10RB
• Synonyms: IL10RB; interleukin 10 receptor, beta; CRFB4, D21S58, D21S66; interleukin-10 receptor subunit beta; CDW210B; CRF2 4; IL 10R2; IL-10RB; IL-10R subunit 2; IL-10R subunit beta; IL-10 receptor subunit beta; cytokine receptor class-II CRF2-4; interleukin-10 receptor subunit 2; cytokine receptor class-II member 4; cytokine receptor family 2 member 4; cytokine receptor family II, member 4; CRFB4; CRF2-4; D21S58; D21S66; IL-10R2
• Gene ID: 3588
• mRNA Refseq: NM_000628
• protein Refseq: NP_000619
• MIM: 123889