Active Recombinant Human IL11 protein, His-tagged

• Cat.No.: IL11-1583H
• Product Overview: Recombinant Human IL11 protein(Pro22-Leu199), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-199 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized ActiveMax Human IL-11, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-11 R alpha, Fc Tag with a linear range of 1-10 ng/mL.The Human IL-11 R alpha (Luc) HEK293 er Cell was stimulated with serial dilutions of ActiveMax Human IL-11, His Tag. The max induction fold was approximately 31.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 21.0 kDa. The protein migrates as 22-24 kDa and 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Pro22-Leu199
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL11 interleukin 11 [ Homo sapiens ]
• Official Symbol: IL11
• Synonyms: IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809