Active Recombinant Human IL12 protein, His-tagged

Cat.No. : IL12-159H
Product Overview : Recombinant Human IL12 protein is produced by HEK293 expression system and is fused with a His tag at the C-terminal. A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.
2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC.
The ED50 for this effect is typically 0.1-0.6 ng/mL.
Molecular Mass : 60.2 kDa
Protein length : IL12A: Met 1-Ser 219
IL12B: Met 1-Ser 328
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/ml.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ] & IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
Official Symbol : IL12A & IL12B
Synonyms : IL12A; NKSF1; CLMF; p35; IL 12; subunit p35; IL 12A; NFSK; CLMF p35; P35; IL-12A; IL12B; NKSF2; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; NKSF; CLMF p40
Gene ID : 3592 & 3593
mRNA Refseq : NM_000882 & NM_002187
Protein Refseq : NP_000873 & NP_002178
MIM : 161560 & 161561
UniProt ID : P29459 & P29460

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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Customer Reviews (3)

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    Effective in Th1 cell differentiation.


      High specificity in ELISA.


        Stable under storage.

        Q&As (10)

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        How does fluorescence microscopy track IL12 internalization and intracellular trafficking? 11/21/2022

        Fluorescence microscopy tracks IL12 internalization and trafficking.

        What techniques dissect IL12's signaling cascades and crosstalk with other cytokines? 11/13/2022

        Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

        What strategies assess IL12's polarization of T cell subsets and cytokine profiles? 01/30/2022

        Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

        How are reporter gene assays tailored to study IL12's promoter activity and response elements? 01/27/2022

        Reporter gene assays analyze IL12's promoter activity and response elements.

        What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide? 09/02/2021

        Super-resolution imaging details IL12's cellular distribution and dynamics.

        What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments? 03/31/2021

        3D cell culture models reveal IL12's effects on tissue microenvironments.

        How do protein-protein interaction assays decipher IL12's binding partners in cellular context? 02/22/2021

        Co-localization studies and co-IP assays identify IL12's binding partners.

        What methods elucidate IL12's receptor complex formation and stoichiometry? 12/18/2020

        Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

        Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks? 10/10/2020

        ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

        Could you explain how transgenic models help dissect IL12's roles in immune response modulation? 03/19/2020

        Transgenic models elucidate IL12's roles in immune response regulation.

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