Active Recombinant Human IL17F Protein (133 aa)

Cat.No. : IL17F-099I
Product Overview : Recombinant Human IL17F Protein without tag was expressed in E. coli.
  • Specification
  • Gene Information
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Description : Human IL-17F is synthesized as a 153 aa precursor with a 20 aa signal sequence and a 133 aa mature region. Like IL-17A, IL-17F contains one potential site for N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. IL17-F homodimer is produced by an activated subset of CD4+ T cells, termed Th17. IL17-F has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Source : E. coli
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Measured by its ability to induce IL-6 production by NHDF cells, it’s fully biologically active when compared to standard.
Molecular Mass : A disulfide-linked homodimer of 30.1kDa, consisting of two 133 amino acid polypeptide chains.
Protein Length : 133
AA Sequence : MRKIPKVGHTFFQKPESCPPVPGGS MKLDIGIINENQRVSMSRNIESRST SPWNYTVTWDPNRYPSEVVQAQCRN LGCINAQGKEDISMNSVPIQQETLV VRRKHQGCSVSFQLEKVLVTVGCTC VTPVIHHVQ
Endotoxin : Less than 1 EU/μg of rHuIL-17F as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IL17F interleukin 17F [ Homo sapiens ]
Official Symbol : IL17F
Synonyms : IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F;
Gene ID : 112744
mRNA Refseq : NM_052872
Protein Refseq : NP_443104
MIM : 606496
UniProt ID : Q96PD4

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques are used to assess IL17F's involvement in cancer development and its potential as a therapeutic target? 03/05/2022

Tumor xenografts, immune cell infiltration analysis, and therapeutic antibody testing assess IL17F's role in cancer.

What methodologies are employed to explore IL17F's role in promoting neutrophil recruitment and activation at mucosal sites? 04/05/2021

Neutrophil chemotaxis assays, in vivo neutrophil tracking, and immune cell activation analysis explore IL17F's impact on neutrophil functions.

How do researchers differentiate the functional roles of IL17F from other IL17 family members in immune responses? 02/04/2021

Genetic knockout models, functional assays, and gene expression analyses distinguish IL17F's unique roles.

How is the interplay between IL17F and gut microbiota investigated, and what are the implications for intestinal health? 07/21/2020

Microbiota composition analysis, germ-free models, and immune cell profiling investigate IL17F-microbiota interactions.

Could you elaborate on the strategies employed to investigate IL17F's contribution to autoimmune diseases using experimental models and patient samples? 05/27/2020

Animal disease models, patient samples, and immunological assays elucidate IL17F's contribution to autoimmune diseases.

What experimental techniques are utilized to dissect the complex heterodimerization of IL17F with IL17A and its impact on signaling pathways? 04/09/2020

Co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and pathway-specific assays elucidate IL17F's heterodimerization and signaling.

How do researchers investigate the potential synergistic or antagonistic effects of IL17F on tissue inflammation in comparison to other cytokines? 12/21/2019

Comparative cytokine profiling, inflammatory assays, and pathway analysis dissect IL17F's interplay with other immune mediators.

What molecular approaches are used to study IL17F's involvement in chronic inflammatory conditions, such as asthma and inflammatory bowel diseases? 03/31/2018

Allergen challenges, inflammatory disease models, and gene expression profiling probe IL17F's involvement in chronic conditions.

Can you explain the experimental designs used to study the impact of IL17F on barrier function in epithelial tissues? 06/24/2017

Epithelial barrier permeability assays, tissue section analysis, and cell culture models study IL17F's effects on barrier integrity.

How do researchers explore the effects of IL17F modulation on tissue repair and regeneration in experimental models? 03/21/2017

Wound healing assays, tissue regeneration models, and histological analyses examine IL17F's effects on tissue repair.

Customer Reviews (3)

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Reviews
11/01/2022

    High specificity. -

    02/23/2020

      Good for receptor studies. -

      02/05/2018

        Potent in anti-bacterial assays. -

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