Active Recombinant Human IL17RA protein, Fc-tagged IL17RA-373H

Active Recombinant Human IL17RA protein, Fc-tagged

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Cat.No. : IL17RA-373H Optional Service: Optional requirements on this protein
Product Overview : Human IL-17 RA, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # NP_055154.3).
Description : Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : Leu 33
Form : Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, 10 mM NaCl, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human IL17 /IL17A,Tag Free at 2 μg/mL (100 μL/well ) can bind Human IL-17 RA, Fc Tag with a linear ranger of 0.3 - 5 ng/mL.
Molecular Mass : This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.1 kDa. The protein migrates as 71-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Leu 33 - Trp 320
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Storage buffer : 50 mM tris, 100 mM glycine, 10 mM NaCl, pH7.5
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : Powder,RT
SDS-PAGE :

Human IL-17 RA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : IL17RA interleukin 17 receptor A [ Homo sapiens (human) ]
Official Symbol : IL17RA
Synonyms : CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R; IL-17 receptor A
Gene ID : 23765
mRNA Refseq : NM_001289905.1
Protein Refseq : NP_001276834.1
UniProt ID : Q96F46
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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