Cat.No. : |
IL17RA-373H
Optional Service: Optional requirements on this protein
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Product Overview : |
Human IL-17 RA, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # NP_055154.3). |
Description : |
Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5). |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Fc |
Predicted N Terminal : |
Leu 33 |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, 10 mM NaCl, pH7.5, 10% trehalose. |
Bio-activity : |
Immobilized Human IL17 /IL17A,Tag Free at 2 μg/mL (100 μL/well ) can bind Human IL-17 RA, Fc Tag with a linear ranger of 0.3 - 5 ng/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.1 kDa. The protein migrates as 71-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : |
Leu 33 - Trp 320 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Storage buffer : |
50 mM tris, 100 mM glycine, 10 mM NaCl, pH7.5 |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
Shipping : |
Powder,RT |
SDS-PAGE : |
Human IL-17 RA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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