Recombinant Cynomolgus IL17RA cell lysate
|Product Overview :||Cynomolgus IL17RA / IL17R derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
- Gene Information
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|Source :||Human cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Surface plasmon resonance, co-immunoprecipitation, and mutagenesis studies reveal IL17RA's binding partners and affinity.
Cross-pathway signaling assays, gene expression profiling, and functional rescue experiments unveil IL17RA's interaction networks.
Angiogenesis assays, fibroblast activation assays, and tissue remodeling models illuminate IL17RA's role in tissue repair.
Animal models, pharmacological interventions, and toxicity assessments evaluate IL17RA-targeted therapies and translational challenges.
Patient cohorts, clinical data analysis, and immune profiling provide insights into IL17RA's relevance in autoimmune diseases.
Flow cytometry, in vivo imaging, and chemotaxis assays characterize immune cell responses guided by IL17RA activation.
Phosphorylation assays, gene expression analysis, and knockout models dissect IL17RA-mediated signaling cascades.
Disease models, antibody-based treatments, and inflammatory biomarker assessments elucidate IL17RA's therapeutic potential.
ChIP-sequencing, promoter analysis, and epigenetic modification studies uncover IL17RA gene regulation mechanisms.
Epithelial barrier assays, microbiome analysis, and infection models decipher IL17RA's role in mucosal defense.
Customer Reviews (3)Write a review
Effective in T-cell activation. -
Suitable for co-immunoprecipitation. -
High solubility. -
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