Active Recombinant Human IL1A Protein

• Cat.No.: IL1A-049H
• Product Overview: Recombinant human interleukin-1α is expressed in E. coli and consists of 159 amino acids with no disulfide bonds.

Specification

• Species: Human
• Source: E.coli
• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
• Form: Sterile lyophilized powder
• Molecular Mass: ~18.0 kDa
• Bio-activity: Specific activity is determined by an IL-1α-dependent proliferation assay using the D10.G4.1 cell line, and is ≥1.6 × 10^8 IU/mg.
• Endotoxin: <1 EU/μg determined by LAL.
• Purity: ≥97%
• Storage: Lyophilized samples remain stable for 36 months when stored at –20 to –70 centigrade from the date of manufacture. After reconstitution, the cytokine can be kept for one month at 2–8 centigrade under sterile conditions, or for three months at –20 to –70 centigrade in a manual-defrost freezer, without significant loss of activity. Avoid repeated freeze-thaw cycles.
• Storage Buffer: This product is supplied as a sterile lyophilized powder in a buffer of PBS, pH7.4, 0.1% HSA.
• Reconstitution: Before opening the vial, centrifuge at 10, 000 rpm for 30–60 seconds, or at 3, 000 rpm for 5 minutes, then reconstitute with 0.2 mL of sterile water.

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Homo sapiens (human) ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA
• Gene ID: 3552
• mRNA Refseq: NM_000575
• Protein Refseq: NP_000566
• MIM: 147760
• UniProt ID: P01583