Active Recombinant Human IL1A protein, Fc-tagged

• Cat.No.: IL1A-4232H
• Product Overview: Active Recombinant Human IL1A protein(P01583)(Phe113-Thr271) is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 113-271 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Human IL-1R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-1 alpha, hFc Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 43.99 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Homo sapiens ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA
• Gene ID: 3552
• mRNA Refseq: NM_000575
• Protein Refseq: NP_000566
• MIM: 147760
• UniProt ID: P01583