Active Recombinant Human IL23A&IL12B protein

• Cat.No.: IL23A&IL12B-3675H
• Product Overview: Recombinant Human IL23A&IL12B protein(23-328 aa & 21-189 aa) was expressed in Sf 21 insect cell.

Specification

• Species: Human
• Source: Sf 21 insect cell
• Tag: Non
• Protein Length: 23-328 aa & 21-189 aa
• Form: Lyophilized from 0.2 μm filtered concentrated sterile solution in 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA.
• Bio-activity: Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/mL.
• Molecular Mass: Apparent molecular mass of 65 kDa, 43-45 kDa and 20 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: Less than 1.0 EU/µg of rHuIL-23 as determined by LAL method.
• Purity: > 95 % by SDS-PAGE analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.