Active Recombinant Human IL2RA&IL2RB&IL2RG protein, Fc-tagged

• Cat.No.: IL2RA&IL2RB&IL2RG-0202H
• Product Overview: Recombinant Human IL2RA&IL2RB&IL2RG protein(Ala27-Asp239&Glu22-Ser212&Leu23-Asn254), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 27-239;22-212;23-254 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Human IL-2, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag with a linear range of 0.4-6 ng/mL.Immobilized Cynomolgus / Rhesus macaque IL-15, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag with a linear range of 0.078-1.25 μg/mL.
• Molecular Mass: The reducing (R) protein migrates as 60-66 kDa and 100-116 kDa respectively due to glycosylation.
• Proteinlength: Ala27-Asp239&Glu22-Ser212&Leu23-Asn254
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2RA interleukin 2 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL2RA
• Synonyms: IL2RA; interleukin 2 receptor, alpha; IL2R; interleukin-2 receptor subunit alpha; CD25; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; TCGFR; IDDM10
• Gene ID: 3559
• mRNA Refseq: NM_000417
• Protein Refseq: NP_000408
• MIM: 147730
• UniProt ID: P01589