Active Recombinant Human IL4

• Cat.No.: IL4-2755H
• Product Overview: Recombinant human IL-4 produced in Yeast is a single, glycosylated, polypeptide chain containing 129 amino acids, three pairs of disulfide bonds and having a molecular mass of approximately 15.0kDa (after glycosylation, the molecular mass is 30.0kDa.)

Specification

• Description: Interleukin 4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. It was initially identified as a B cell differentiation factor (BCDF), as well as a B cell stimulatory factor (BSF1). Subsequent to the molecular cloning and expression of both human and Mouse IL-4, numerous other functions have been described on B cells as well as other hematopoietic and nonhematopoietic cells, including T lymphocytes, monocytes, macrophages, mast cells, myeloid and erythroid progenitors, fibroblasts, endothelial cells, etc. IL-4 exhibits anti-tumor effects both in vivo and in vitro.
• Source: Yeast
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 20mM Phosphate Buffer
• Bio-activity: Measured in a cell proliferation assay using a human factor-dependent cell line, TF-1. The specific activity shall be not less than 1×10^7 IU/mg.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2- 8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 5μg/ml of the cytokine.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Official Symbol: IL4
• Synonyms: BSF1; IL-4; BCGF1; BSF-1; BCGF-1; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112
• Chromosome Location: 5q31.1
• Pathway: Allograft Rejection, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
• Function: cytokine activity