Species : |
Human |
Source : |
Yeast |
Tag : |
Non |
Description : |
Interleukin 4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. It was initially identified as a B cell differentiation factor (BCDF), as well as a B cell stimulatory factor (BSF1). Subsequent to the molecular cloning and expression of both human and Mouse IL-4, numerous other functions have been described on B cells as well as other hematopoietic and nonhematopoietic cells, including T lymphocytes, monocytes, macrophages, mast cells, myeloid and erythroid progenitors, fibroblasts, endothelial cells, etc. IL-4 exhibits anti-tumor effects both in vivo and in vitro. |
Form : |
Lyophilized from a 0.2μm filtered solution in 20mM Phosphate Buffer |
Bio-activity : |
Measured in a cell proliferation assay using a human factor-dependent cell line, TF-1. The specific activity shall be not less than 1×10^7 IU/mg. |
Endotoxin : |
≤1EU/μg, determined by the LAL method. |
Purity : |
≥97%, as determined by SDS-PAGE and HPLC method. |
Usage : |
FOR RESEARCH ONLY |
Storage : |
Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2- 8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 5μg/ml of the cytokine. |