Active Recombinant Human IL4,His-tagged
Cat.No. : | IL4-8863H |
Product Overview : | Recombinant Human IL4 was produced in the endosperm tissue of barley grain (Hordeum vulgare). |
- Specification
- Gene Information
- Related Products
Description : | IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells. |
Source : | Hordeum vulgare |
Species : | Human |
Predicted N Terminal : | His |
Form : | Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH7,2 |
Bio-activity : | The bioactivity of recombinant human IL-4 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically = 0.2 ng/ml, corresponding to a specific activity of = 5 x 10e6 units/mg. |
Molecular Mass : | Recombinant human IL-4 contains 130 amino acids and a 16 a.a. Histidine-based tag for a total length of 146 a.a. and has a predicted molecular mass of 17.2 kDa. The recombinant protein migrates as two bands with an apparent molecular mass of 23 kDa and 24 kDa in SDS-PAGE. |
Endotoxin : | Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay. |
Purity : | >95% by SDS-PAGE gel analysis. |
Storage : | The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Gene Name : | IL4 interleukin 4 [ Homo sapiens ] |
Official Symbol : | IL4 |
Synonyms : | IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1; |
Gene ID : | 3565 |
mRNA Refseq : | NM_000589 |
Protein Refseq : | NP_000580 |
MIM : | 147780 |
UniProt ID : | P05112 |
Chromosome Location : | 5q23-q31 |
Pathway : | Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD40/CD40L signaling, organism-specific biosystem; |
Function : | cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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