Active Recombinant Human IL6R, His-tagged

• Cat.No.: IL6R-428H
• Product Overview: Recombinant Human IL6R (Accession # ACC04617) Ala22-Val346, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 22-346 a.a.
• Predicted N Terminal: Ala22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 4-20 µg/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human IL6R, His-tagged has a calculated MW of 38.9 kDa. In SDS-PAGE migrates as 55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 250 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL6R interleukin 6 receptor [ Homo sapiens ]
• Official Symbol: IL6R
• Synonyms: IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1; MGC104991
• Gene ID: 3570
• mRNA Refseq: NM_000565
• Protein Refseq: NP_000556
• MIM: 147880
• UniProt ID: P08887
• Chromosome Location: 1q21