| Species : |
Human |
| Source : |
Hordeum Vulgare |
| Tag : |
Non |
| Description : |
IL-9 is a cytokine with immunoregulatory function, produced by activated T-helper type 2 lymphocytes. It acts on various cell types such as T- and B-lymphocytes, mast cells, haemopoietic progenitors and megakaryoblastic leukemia cell lines. IL-9 stimulates cell proliferation and prevents apoptosis. IL-9 may play a role in the pathogenesis of diseases such as certain lymphomas (Hodgin′s disease, anaplastic lymphoma) and asthma. |
| Predicted N Terminal : |
His |
| Form : |
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS |
| Bio-activity : |
Bioactivity is assayed by measuring the dose dependent effect of human IL-9 on proliferation of murine TS1h9RA3 cell line, transfected with human IL-9 receptor (Renauld & Van Snick, Curr. Prot. in Immunol.2002). The ED50 of recombinant human IL-9 is 120 pg/ml. |
| Molecular Mass : |
Recombinant human IL-9 contains 127 amino acids and a 16 a.a. histidine-based tag for a total length of 143 a.a. and has a predicted molecular mass of 16.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as 3 bands with an apparent molecular mass of 20, 22 and 24 kDa in SDS-PAGE. |
| Endotoxin : |
Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay. |
| Purity : |
>95% by SDS-PAGE gel analysis. |
| Storage : |
The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
