Active Recombinant Human INS protein

• Cat.No.: INS-320H
• Product Overview: Active Recombinant Human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed in Yeast with a linker AAK.

Specification

• Species: Human
• Source: Yeast
• Tag: Non
• Protein Length: 1-29 aa & 1-21 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured in a serum-free cell liferation assay using MCF7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 μg/mL.
• Molecular Mass: The recombinant human Insulin consists of 63 amino acids and predicts a molecular mass of 7 KDa.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000198.1
• Unit ID: P01308
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P01308

Gene Information

• Gene Name: INS insulin [ Homo sapiens ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207
• protein Refseq: NP_000198