Active Recombinant Human INS protein

• Cat.No.: INS-0301H
• Product Overview: Recombinant Human INS protein(P01308)(Phe25-Lys53 & Gly90-Asn110) was expressed in Yeast.

Specification

• Species: Human
• Source: Yeast
• Tag: Non
• Protein Length: 25-53;90-110 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 µg/mL.
• Molecular Mass: The recombinant human Insulin consists of 63 amino acids and predicts a molecular mass of 7.1 KDa. It migrates as an approximately 6 KDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Phe25-Lys53 & Gly90-Asn110
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: INS insulin [ Homo sapiens ]
• Official Symbol: INS
• Synonyms: INS; insulin; proinsulin; ILPR; IRDN; IDDM2; MODY10
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• UniProt ID: P01308