Active Recombinant Human IRE1 protein, His&GST-tagged

• Cat.No.: ERN1-1124H
• Product Overview: Recombinant Human IRE1 protein(O75460-1)(Pro465-Leu977), fused with His tag and GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Supplied as sterile 20mM Tris, 500M NaCl, 25% glycerol, 0.1mM EDTA, 0.5mM TCEP, 2mM GSH, pH 7.5.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
• Molecular Mass: The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Pro465-Leu977
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Pro465-Leu977

Citation

Regulated IRE1α-dependent decay (RIDD)-mediated reprograming of lipid metabolism in cancer

Journal: Nature Communications    PubMed ID: 35524156    Data: 2022/5/6

Authors: Aitor Almanza, Katarzyna Mnich, Afshin Samali

Article Snippet:RNA was purified with Nucleospin PCR cleanup system.RNA was purified with Nucleospin PCR cleanup system.. RNA (1 μg) was incubated with recombinant IRE1α-N-His-GST fragment containing the cytosolic kinase and endoribonuclease domains (aa 465-977) (Creative BioMart, ERN1-1124H) in the presence or absence of 20 μM MKC8866 for 1 h at 37 °C in a buffer containing 40 mM HEPES pH 7.5, 2 mM MgOAc 2 , 100 mM KOAc, 1 mM DTT.. Products of the digestion were separated in a 1.5% agarose gel made in 1× MOPS (Fisher Scientific, BP2900-1) and visualized with DDGIT gel scanner (LI-COR).Products of the digestion were separated in a 1.5% agarose gel made in 1× MOPS (Fisher Scientific, BP2900-1) and visualized with DDGIT gel scanner (LI-COR).

Publication :

Regulated IRE1α-dependent decay (RIDD)-mediated reprograming of lipid metabolism in cancer (2022)