Active Recombinant Human ITGA4 & ITGB1 Protein, His-Avi-tagged, Biotinylated

Cat.No. : ITGA4 & ITGB1-162H
Product Overview : Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein is expressed from HEK293 cells. It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
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Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Tyr 34 (ITGA4) & Gln 21 (ITGB1)
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Natalizumab at 1 ug/mL (100 uL/well) can bind Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein with a linear range of 2-31 ug/mL.
Molecular Mass : Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) & 83.7 kDa (ITGB1). The reducing protein migrates as 100-150 kDa (ITGA4) and 65-85 kDa (ITGB1) respectively due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Official Symbol : ITGA4; ITGB1
Synonyms : Integrin alpha 4 beta 1, ITGA4 & ITGB1
Official Symbol : ITGA4; ITGB1
Synonyms : Integrin alpha 4 beta 1, ITGA4 & ITGB1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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