Active Recombinant Human ITGA4/ITGB7 protein

• Cat.No.: ITGA4/ITGB7-356H
• Product Overview: Recombinant Human ITGA4(Tyr34-Gln970)/ITGB7(Glu20-His723) was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Predicted N Terminal: Tyr34 (Integrin alpha 4), Glu20 (Integrin beta 7)
• Form: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and MgCl2.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 10^4 cells per well are added to Recombinant Human Integrin alpha 4/ beta 7/LPAM-1 coated plates (10 ug/mL, 100uL/well), approximately 50-80% will adhere after 60 minutes at 37 centigrade.
• Molecular Mass: Predicted Molecular Mass: 112.8 (Integrin alpha 4) & 84.7 (Integrin beta 7) kDa;SDS-PAGE: 150-170 kDa and 125-135 kDa
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 ug/mL in sterile PBS.

Gene Information

• Gene Name: ITGA4 integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) [ Homo sapiens ]
• Official Symbol: ITGA4
• Synonyms: ITGA4; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); CD49D; integrin alpha-4; CD49d; 269C wild type; integrin alpha 4; integrin alpha-IV; VLA-4 subunit alpha; integrin alpha-4 subunit; CD49 antigen-like family member D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; very late activation protein 4 receptor, alpha 4 subunit; IA4; MGC90518
• Gene ID: 3676
• mRNA Refseq: NM_000885
• Protein Refseq: NP_000876
• MIM: 192975
• UniProt ID: P13612
• Chromosome Location: 2q31-q32
• Pathway: Adaptive Immune System, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Dilated cardiomyopathy, organism-specific biosystem
• Function: fibronectin binding; protein binding; receptor activity