Active Recombinant Human ITGAL&ITGB2 protein, His-tagged

• Cat.No.: ITGAL&ITGB2-0223H
• Product Overview: Recombinant Human ITGAL&ITGB2 protein(Tyr26-Met1089(ITGAL)&Gln23-Asn700(ITGB2)), fused with His tag, was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free at 10 μg/mL (100 μL/well) can bind Human ICAM-1, Fc Tag with a linear range of 0.4-6 ng/mL.Immobilized Human ICAM-1, Fc Tag at 10 μg/mL (100 μL/well) can bind Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.156-5 μg/mL.
• Molecular Mass: The protein migrates as 140-150 kDa (ITGAL) and 90-95 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Tyr26-Met1089(ITGAL)&Gln23-Asn700(ITGB2)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGAL integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) [ Homo sapiens ]
• Official Symbol: ITGAL
• Synonyms: ITGAL; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); CD11A; integrin alpha-L; LFA 1; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; LFA-1; LFA1A
• Gene ID: 3683
• mRNA Refseq: NM_001114380
• Protein Refseq: NP_001107852
• MIM: 153370
• UniProt ID: P20701