Active Recombinant Human KLK12 protein, His-tagged, Low Endotoxin

• Cat.No.: KLK12-1977H
• Product Overview: Recombinant Human KLK12 protein(Q9UKR0-1)(Ile22-Asn248), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-248 aa
• Form: Lyophilized from 0.22 μm filtered solution in 20mM NaAC, 150mM NaCl, 8% trehalose, 0.05% Brij 35, 10mM Benzamidine (pH 5.0).
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >15000 pmol/min/μg.
• Molecular Mass: The protein has a predicted MW of 26.18 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK12 kallikrein-related peptidase 12 [ Homo sapiens ]
• Official Symbol: KLK12
• Synonyms: KLK12; kallikrein-related peptidase 12; kallikrein 12; kallikrein-12; KLK L5; kallikrein-like protein 5; KLKL5; KLK-L5; MGC42603; DKFZp686H1078
• Gene ID: 43849
• mRNA Refseq: NM_019598
• Protein Refseq: NP_062544
• MIM: 605539
• UniProt ID: Q9UKR0