Active Recombinant Human KLK4 protein, His-tagged, Low Endotoxin

• Cat.No.: KLK4-1169H
• Product Overview: Low Endotoxin Active Recombinant Human KLK4 protein(Q9Y5K2-1)(Ile31-Ser254) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 31-254 aa
• Form: Lyophilized from 0.22 μm filtered solution in 20mM HEPES, 150mM NaCl, 8% trehalose, 0.05% Brij-35, 5mM Benzamidine (pH 7.5).
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >1200 pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 25.61 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK4 kallikrein-related peptidase 4 [ Homo sapiens ]
• Official Symbol: KLK4
• Synonyms: KLK4; kallikrein-related peptidase 4; kallikrein 4 (prostase, enamel matrix, prostate), PRSS17; kallikrein-4; EMSP; EMSP1; KLK L1; PSTS; prostase; serine protease 17; kallikrein-like protein 1; androgen-regulated message 1; enamel matrix serine protease 1; enamel matrix serine proteinase 1; ARM1; AI2A1; KLK-L1; PRSS17; kallikrein; MGC116827; MGC116828
• Gene ID: 9622
• mRNA Refseq: NM_004917
• Protein Refseq: NP_004908
• MIM: 603767
• UniProt ID: Q9Y5K2