Active Recombinant Human KLK5 protein, GGS-8H-tagged

• Cat.No.: KLK5-27H
• Product Overview: Recombinant Human KLK5 protein (Ile67- Ser293) (Accession: # Q9Y337), fused with a C-terminal GGS-8H tag was expressed in CHO cells.

Specification

• Species: Human
• Source: CHO
• Protein Length: Ile67- Ser293
• Form: 0.22 µm filtered protein solution is in 25 mM MES, 150 mM NaCl, 20% glycerol pH 5.5
• Bio-activity: Recombinant human KLK5 activity is measured by its ability to cleave the fluorogenic peptide substrate Boc-Val-Pro-Arg-AMC. The specific activity is > 200 pmol/min/µg in the presence of 0.1 µg of recombinant human KLK5.
• Molecular Mass: The 236 amino acid recombinant protein has a predicted molecular mass of approximately 26.1 kD. The DTT-reduced and non-reduced protein migrates at approximately 60 and 50 kD by SDS-PAGE respectively.
• Endotoxin: Less than 0.1 EU per µg cytokine as determined by the LAL method
• Purity: > 95%, as determined by Coomassie stained SDS-PAGE
• Storage: Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.
• Concentration: 10 and 25 µg sizes are bottled at 200 µg/mL.

Gene Information

• Gene Name: KLK5 kallikrein related peptidase 5 [ Homo sapiens (human) ]
• Official Symbol: 25818
• Synonyms: SCTE; KLKL2; KLK-L2
• Gene ID: 25818
• mRNA Refseq: NM_001077491.2
• Protein Refseq: NP_001070959.1
• MIM: 605643
• UniProt ID: Q9Y337