Active Recombinant Human KLK5 protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: KLK5-3234H
• Product Overview: Recombinant Human KLK5 protein(Q9Y337)(Ile67-Ser293), fused with C-terminal His and Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 67-293 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 31.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK5 kallikrein-related peptidase 5 [ Homo sapiens ]
• Official Symbol: KLK5
• Synonyms: KLK5; kallikrein-related peptidase 5; kallikrein 5; kallikrein-5; KLK L2; SCTE; kallikrein-like protein 2; stratum corneum tryptic enzyme; KLKL2; KLK-L2
• Gene ID: 25818
• mRNA Refseq: NM_001077491
• Protein Refseq: NP_001070959
• MIM: 605643
• UniProt ID: Q9Y337