Active Recombinant Human KLK5 protein, His-tagged

• Cat.No.: KLK5-25H
• Product Overview: Recombinant Human KLK5(Ile67-Ser293) fused with His tag at C-terminal was expressed in NS0.

Specification

• Description: Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family. The deduced amino acid sequence of human KLK5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). KLK5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues. KLK5 overexpression is an indicator of poor prognosis in ovarian cancer. hK5 is a candidate physiological activator of hK7 in the stratum corneum. Indeed, rhK5 can be used to activate rhK7 by incubating rhK5 with recombinant human (rh) K7 (both at 0.1 mg/mL) in 25 mM MES, 0.15 M NaCl, pH 5.5, at 37 centigrade overnight.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ile67
• Form: Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 31 kDa;
  SDS-PAGE: 40 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: KLK5 kallikrein-related peptidase 5 [ Homo sapiens ]
• Official Symbol: KLK5
• Synonyms: KLK5; kallikrein-related peptidase 5; kallikrein 5; kallikrein-5; KLK L2; SCTE; kallikrein-like protein 2; stratum corneum tryptic enzyme; KLKL2; KLK-L2
• Gene ID: 25818
• mRNA Refseq: NM_001077491
• Protein Refseq: NP_001070959
• MIM: 605643
• UniProt ID: Q9Y337
• Chromosome Location: 19q13.33
• Function: peptidase activity; protein binding; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity