Active Recombinant Human LILRB4 protein, His-Avi-tagged, Biotinylated

• Cat.No.: LILRB4-0337H
• Product Overview: Biotinylated Recombinant Human LILRB4 protein(Gln22-Glu259), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human LILRB4, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-LILRB4 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 29.8 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln22-Glu259
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LILRB4 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 [ Homo sapiens ]
• Official Symbol: LILRB4
• Synonyms: HM18; ILT3; LIR5; CD85K; LIR-5; LILRB5
• Gene ID: 11006
• mRNA Refseq: NM_006847.3
• Protein Refseq: NP_006838.3
• MIM: 604821
• UniProt ID: Q8NHJ6