Active Recombinant Human LRP6 protein, His-Avi-tagged, Biotinylated

• Cat.No.: LRP6-0371H
• Product Overview: Biotinylated Recombinant Human LRP6 protein(Ala20-Pro630), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-630 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human R-Spondin 1, His Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human LRP-6, His,Avitag with a linear range of 20-156 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala20-Pro630
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: LRP6 low density lipoprotein receptor-related protein 6 [ Homo sapiens ]
• Official Symbol: LRP6
• Synonyms: LRP6; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; ADCAD2; FLJ90062; FLJ90421
• Gene ID: 4040
• mRNA Refseq: NM_002336
• Protein Refseq: NP_002327
• MIM: 603507
• UniProt ID: O75581