Active Recombinant Human MAP1LC3A Protein, His-tagged
Cat.No. : | MAP1LC3A-784H |
Product Overview : | Recombinant Human MAP1LC3A (Accession # NP_115903), fused with an N-terminal 6-His tag, was produced in E. coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Form : | X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl.Actual concentration varies with lot number. |
Bio-activity : | Biotin-His6-LC3/MAP1LC3A/Apg8p3 can be conjugated to substrate proteins via the subsequent actions of a Apg-activating (E1) enzyme, a Apg-conjugating (E2) enzyme, and a Apg ligase (E3). Biotin-His6-LC3/MAP1LC3A/Apg8p3 is ideal for the visualization or quantification of thioester formation with avidin-linked reagents. Reaction conditions will need to be optimized for each specific application. We recommend an initial Biotin-His6-LC3/MAP1LC3A/Apg8p3 concentration of 1-5 µM. |
Purity : | >95%, by PAGE. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name | MAP1LC3A microtubule-associated protein 1 light chain 3 alpha [ Homo sapiens ] |
Official Symbol | MAP1LC3A |
Synonyms | MAP1LC3A; microtubule-associated protein 1 light chain 3 alpha; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; |
Gene ID | 8199 |
mRNA Refseq | |
Protein Refseq | |
MIM | 601242 |
UniProt ID | Q9H492 |
Chromosome Location | 20q11.22 |
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Not For Human Consumption!
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