Active Recombinant Human MAP1LC3A Protein, His-tagged

• Cat.No.: MAP1LC3A-784H
• Product Overview: Recombinant Human MAP1LC3A (Accession # NP_115903), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (XµM) in 50mM Hepes pH 7.5, 100mM NaCl.Actual concentration varies with lot number.
• Bio-activity: Biotin-His6-LC3/MAP1LC3A/Apg8p3 can be conjugated to substrate proteins via the subsequent actions of a Apg-activating (E1) enzyme, a Apg-conjugating (E2) enzyme, and a Apg ligase (E3). Biotin-His6-LC3/MAP1LC3A/Apg8p3 is ideal for the visualization or quantification of thioester formation with avidin-linked reagents. Reaction conditions will need to be optimized for each specific application. We recommend an initial Biotin-His6-LC3/MAP1LC3A/Apg8p3 concentration of 1-5 µM.
• Purity: >95%, by PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MAP1LC3A microtubule-associated protein 1 light chain 3 alpha [ Homo sapiens ]
• Official Symbol: MAP1LC3A
• Synonyms: MAP1LC3A; microtubule-associated protein 1 light chain 3 alpha; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
• Gene ID: 8199
• MIM: 601242
• UniProt ID: Q9H492
• Chromosome Location: 20q11.22