Active Recombinant Human Full Length MMP8 Protein, His-tagged, Low Endotoxin

• Cat.No.: MMP8-2529H
• Product Overview: Active Recombinant Human Full Length MMP8 Protein(NP_002415.1) (Met 1-Gly 467) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-467 aa
• Bio-activity: 1.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 250 pmoles/minμg.(Activation description: The proenzyme needs to be activated by APMA for an activated form) 2.Immobilized Recombinant Human MMP8 / CLG1 Protein (His Tag) at 1 μg/mL (100 μL/well) can bind Anti-MMP-8 Antibody, Rabbit Polyclonal, the EC50 is 1.7 ng/mL.
• Molecular Mass: The recombinant human MMP8 consists of 458 amino acids after removal of the signal peptide and has a predicted molecular mass of 52.6 kDa.
• Endotoxin: < 0.5 EU per mg protein.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MMP8 matrix metallopeptidase 8 (neutrophil collagenase) [ Homo sapiens ]
• Official Symbol: MMP8
• Synonyms: MMP8; matrix metallopeptidase 8 (neutrophil collagenase); CLG1, matrix metalloproteinase 8 (neutrophil collagenase); neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); HNC; CLG1; MMP-8; PMNL-CL
• Gene ID: 4317
• mRNA Refseq: NM_002424
• Protein Refseq: NP_002415
• MIM: 120355
• UniProt ID: P22894