Active Recombinant Human/Mouse WNT5A Protein

• Cat.No.: WNT5A-58H
• Product Overview: Recombinant Human/Mouse WNT5A Protein without tag was expressed in CHO.

Specification

• Description: The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the WNT gene family. Mature mouse Wnt5a is 100% identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibits or activates TCF-based Wnt signaling. This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound Sepharose beads, and followed by gel filtration.
• Source: CHO
• Species: Human/Mouse
• Form: Liquid
• Bio-activity: The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF-based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 50-100 ng/mL in the presence of 1-2 ng/mL of mouse Wnt3a.
• Molecular Mass: ~45 kDa
• Endotoxin: <0.5 EU/ μg
• Purity: High Purity, >75% by SDS-PAGE
• Stability: Keep the protein frozen until use. Freeze at -20 centigrade or below after thawed. Mix the protein by pipetting up and down only, but do not use vortexer.
• Storage Buffer: PBS pH7.4-7.6, 1%Chaps, 0.1% BSA
• Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at -80 centigrade recommended.

Gene Information

• Gene Name: WNT5A Wnt family member 5A [ Homo sapiens (human) ]
• Official Symbol: WNT5A
• Synonyms: WNT5A; Wnt family member 5A; hWNT5A; protein Wnt-5a; WNT-5A protein; epididymis secretory sperm binding protein; wingless-type MMTV integration site family, member 5A
• Gene ID: 7474
• mRNA Refseq: NM_003392
• Protein Refseq: NP_003383
• MIM: 164975
• UniProt ID: P41221

Reviews

11/14/2019

Satisfied with performance, quality.

08/27/2019

Great product, satisfied customer.

02/27/2019

High purity, works well.

Q&As

What role does WNT5A play in cancer biology?

Its role in cancer is complex, acting as a tumor suppressor or promoter depending on context.

How does WNT5A interact with its receptors to initiate signal transduction?

It binds to Frizzled receptors and co-receptors, activating non-canonical Wnt signaling pathways.

In which developmental processes is WNT5A critically involved?

WNT5A is involved in embryonic development, particularly in limb formation and organogenesis.

How is WNT5A implicated in immune system regulation?

WNT5A modulates various immune responses, influencing inflammation and cell migration.

What is the primary role of WNT5A in cellular processes?

WNT5A is crucial in regulating cell proliferation, migration, and differentiation.

What therapeutic strategies are being explored involving WNT5A modulation?

Strategies include developing WNT5A modulators for potential use in cancer therapy and regenerative medicine.

Are there known genetic mutations in WNT5A associated with specific diseases?

Genetic mutations and altered expression of WNT5A are linked to developmental disorders and cancer.