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Active Recombinant Human MSLN Protein

Cat.No. : MSLN-8392H
Product Overview : Recombinant human MSLN is expressed as a 317-amino acid protein consisting of Glu296 - Ser592 region of MSLN (UniProt accession #Q13421, isoform 2) and a C-terminal His-tag. It contains 4 potential N-linked glycosylation sites.
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Source : Human cells
Species : Human
Bio-activity : Binds to CA125/MUC16 and anti-MSLN monoclonal antibody, human IgG1 with high affinity KD < 5 nM as measured by ELISA.
Molecular Mass : Calculated molecular mass (kDa): 35.9; Estimated by SDS-PAGE under reducing condition (kDa): 48-50
AA Sequence : EVEKTACPSGKKAREIDESLIFYKK WELEACVDAALLATQMDRVNAIPFT YEQLDVLKHKLDELYPQGYPESVIQ HLGYLFLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLIDRFVK GRGQLDKDTLDTLTAFYPGYLCSLS PEELSSVPPSSIWAVRPQDLDTCDP RQLDVLYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQNVSMDL ATFMKLRTDAVLPLTVAEVQKLLGP HVEGLKAEERHRPVRDWILRQRQDD LDTLGLGLQGGIPNGYLVLDLSTTE NLYFQGSTGHHHHHHHH
Endotoxin : <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Purity : >90% judged by SDS-PAGE under reducing condition.
Storage : The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Storage Buffer : Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Warning : FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.
Gene Name : MSLN
Official Symbol : MSLN mesothelin [ Homo sapiens (human) ]
Synonyms : MSLN; MPF; SMRP; CAK1; mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
Gene ID : 10232
mRNA Refseq : NM_001177355
Protein Refseq : NP_001170826
MIM : 601051
UniProt ID : Q13421

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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