Active Recombinant Human MUC16 protein, His-tagged

• Cat.No.: MUC16-0273H
• Product Overview: Recombinant Human MUC16 protein(Pro13810-Pro14451), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 13810-14451 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Human MSLN / Mesothelin Protein at 2 μg/ml (100 μl/well) can bind Recombinant Human CA125 Protein (ECD, His Tag), the EC50 is 6-18 ng/mL.
• Molecular Mass: The recombinant human MUC16 consists of 653 amino acids and predicts a molecular mass of 73.27 kDa. It migrates as an approximately 101.25 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Pro13810-Pro14451
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens ]
• Official Symbol: MUC16
• Synonyms: MUC16; mucin 16, cell surface associated; mucin-16; CA125; FLJ14303; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690
• Protein Refseq: NP_078966
• MIM: 606154
• UniProt ID: Q8WXI7