Active Recombinant Human MYC protein, arginine-tagged

• Cat.No.: MYC-79H
• Product Overview: Recombinant human wild-type cMyc cDNA (454 aa) fused with flexible linker domain & eleven arginine (11R Tag) at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: Arginine
• Form: 0.50 mg/ml in sterile-filtered solution in 20 mM Tris, pH 7.5. Proprietary formulation of NaCl , KCl, CaCl2, MgCl2, arginine, DTT, and glycerol.
• Bio-activity: Cellular Toxicity: This recombinant protein was tested on mouse embryonic stem cells up to 50 µg/ml in culture medium. Suggested reprogramming protein concentration is between 0.5 to 8 ug / ml for both human and mouse fibroblast cells applications.Biolog
• AA Sequence: MDFFRVVENQQPPATMPLNVSFTNR NYDLDYDSVQPYFYCDEEENFYQQQ QQSELQPPAPSEDIWKKFELLPTPP LSPSRRSGLCSPSYVAVTPFSLRGD NDGGGGSFSTADQLEMVTELLGGDM VNQSFICDPDDETFIKNIIIQDCMW SGFSAAAKLVSEKLASYQAARKDSG SPNPARGHSVCSTSSLYLQDLSAAA SECIDPSVVFPYPLNDSSSPKSCAS QDSSAFSPSSDSLLSSTESSPQGSP EPLVLHEETPPTTSSDSEEEQEDEE EIDVVSVEKRQAPGKRSESGSPSAG GHSKPPHSPLVLKRCHVSTHQHNYA APPSTRKDYPAAKRVKLDSVRVLRQ ISNNRKCTSPRSSDTEENVKRRTHN VLERQRRNELKRSFFALRDQIPELE NNEKAPKVVILKKATAYILSVQAEE QKLISEEDLLRKRREQLKHKLEQLR NSCAESGGGGSPGRRRRRRRRRRR
• Purity: >93% by SDS-PAGE
• Applications: 1. May be used for in vitro human cMyc mediated iPS generation mechanism, or its gene specific transcription regulation study with intracellular delivery of this protein.2. May be used as specific substrate protein for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.3. May be used for cMyc protein-protein interaction mapping.4. May be used for specific antibody production.
• Storage: Recombinant Human c-Myc-11R is stable for six months at -20centigrade to -80centigrade. Store thawed tube at 4centigrade for seven days. Multiple freeze/thaw cycles may result in significant loss of activity.

Gene Information

• Gene Name: MYC v-myc myelocytomatosis viral oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: MYC
• Synonyms: MYC; v-myc myelocytomatosis viral oncogene homolog (avian); v myc avian myelocytomatosis viral oncogene homolog; myc proto-oncogene protein; bHLHe39; c Myc; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian m
• Gene ID: 4609
• mRNA Refseq: NM_002467
• Protein Refseq: NP_002458
• MIM: 190080
• UniProt ID: P01106
• Chromosome Location: 8q24
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Apoptosis, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem
• Function: DNA binding; DNA binding; E-box binding; protein binding; protein complex binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; transcription factor binding

Reviews

05/19/2022

The reproducibility of MYC is very good, and the results remain consistent even after repeated experiments several times, which gives me more confidence in the experimental results.

01/18/2020

Stability of this protein was still very good when different chemicals or drugs were added, indicating its excellent anti-interference ability and stability.

10/06/2019

Due to stability of MYC in Western blot was very good and even prolonged storage did not affect its performance.

Q&As

How does overexpression of the MYC gene affect cardiovascular disease?

Abnormal expression of MYC gene may affect the occurrence and progression of cardiovascular diseases such as atherosclerosis by regulating the proliferation of vascular smooth muscle cells and increasing the expression of endothelial cell-specific shear-related proteins.

Is the MYC gene a potential target for pancreatic cancer or drug therapy?

Because of its important promotion role in pancreatic cancer, MYC gene is regarded as one of the potential targets and drug treatment directions for pancreatic cancer treatment, but specific research and related drugs need to be further verified.

How does the MYC gene regulate somatic cell proliferation through cell cycle progression?

The MYC gene may affect the proliferation of somatic cells by regulating cell cycle progression, such as increasing the S-phase entry of the cell cycle and promoting the G1/S phase transition.

What is the role of the MYC gene in stomach cancer?

Overexpression and defect of MYC in gastric cancer are associated with the occurrence, progression and prognosis of gastric cancer. MYC translocation and high expression predict a poor prognosis.

Is the MYC gene associated with hematopoietic stem cells?

MYC gene may be involved in the proliferation and differentiation of hematopoietic stem cells, but the specific mechanism and role still need to be further elucidated.

How does abnormal expression of the MYC gene affect tumor progression?

Abnormal expression of MYC gene may affect tumor progression and prognosis by activating cell cycle constants, apoptosis inhibition, and enhanced cell mobility.