| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
Met1-Ser349 |
| Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4 His at 2 μg/ml (100 μl/well) can bind Human PVRL1 His, biotinylated, the EC50 of Human PVRL1 His, biotinylated is 200-800 ng/mL. |
| Molecular Mass : |
The recombinant human NECTIN4 consists of 329 amino acids and predicts a molecular mass of 35.6 kDa. |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
>95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |