| Description : |
Neutrophil gelatinase-associated lipocalin (NGAL) is a 25KD glucoprotein and a new member of lipocalin family with a typical ?-barrel structure of the family. Based on the recent research, the function of NGAL protein may be is to protect and regulate the activity of matrix metalloproteinase-9(MMP-9) and to involve in inflammation and innate immunity reaction. NGAL protein also is a novel iron transporter that mediates a new iron delivery pathway.Acute kidney injury(AKI) is a syndrome characterized by loss of function in a short time ( hours to days ).The scarcity of early biomarker for AKI has hindered our ability to launch the therapy for this disease.Neutrophil gelatinase-assocliated lipocalin( NGAL ) is a high efficient clinical biomarker for AKI. At present , Cystatin C is widely used for AKI clinical diagnosis. However, compared to Cystatin C , NGAL diagnosis value for AKI is much higher . |
| Source : |
E.coli |
| Species : |
Human |
| Predicted N Terminal : |
Gln 21 |
| Form : |
Liquid |
| Bio-activity : |
Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. |
| Molecular Mass : |
The recombinant human NGAL consists of 197 amino acids. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLCN2 is approximately 25 kDa as predicted. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95% as determined by SDS-PAGE |
| Applications : |
It can be used to prepare high titer anti-NGAL antibody . And at the same time the NGAL protein can also be used as positive control in NGAL diagnosis Kit (renal function analysis , AKI analysis). |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening. |
| Concentration : |
1 mg/ml |
| Storage Buffer : |
20 mM Tris pH 7.5, 20% glycerol |
