Active Recombinant Human NGF protein

• Cat.No.: NGF-05H
• Product Overview: Active Recombinant Human NGF protein(NP_002497.2) (Ser 122-Arg 239) was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 122-239 aa
• Form: Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
• Molecular Mass: The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_002497.2
• Unit ID: P01138
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P01138
• Publications:
  • Development of novel HDL-mimicking α-tocopherol-coated nanoparticles to encapsulate nerve growth factor and evaluation of biodistribution. (2016)
  • Non-helical DNA Triplex Forms a Unique Aptamer Scaffold for High Affinity Recognition of Nerve Growth Factor. (2015)
  • Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation (2017)

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
• Official Symbol: NGF
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; beta-nerve growth factor; nerve growth factor, beta subunit; HSAN5; Beta-NGF; MGC161426; MGC161428
• Gene ID: 4803
• mRNA Refseq: NM_002506
• protein Refseq: NP_002497
• MIM: 162030