| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
122-241 aa |
| Description : |
Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant. |
| Specificity : |
Sterility: Negative
Mycoplasma: Negative |
| Bio-activity : |
Immobilized Human Beta-NGF Protein, premium grade at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Mouse IgG2a Fc Tag with a linear range of 1-16 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 13.5 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 0.01 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
