Active Recombinant Human NKG2A & CD94 protein, Fc-tagged

• Cat.No.: NKG2A & CD94-0558H
• Product Overview: Recombinant Human NKG2A & CD94 protein(Ala113-Leu233(NKG2A)&Asp57-Ile179(CD94)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 113-233;57-179 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human NKG2A&CD94, Fc Tag at 2 μg/mL (100 μL/well) can bind Anti-NKG2A Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 55.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala113-Leu233(NKG2A)&Asp57-Ile179(CD94)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.