Active Recombinant Human NODAL Protein (His238-Leu347), C-His tagged, Animal-free, Carrier-free

• Cat.No.: NODAL-235H
• Product Overview: Recombinant Human NODAL Protein (His238-Leu347) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone morphogenetic protein 16 (BMP-16) predicts a molecular mass of 18 kDa. BMPs are multi-functional Growth Factorss that belong to the transforming Growth Factors beta (TGF-β) superfamily. BMPs initiate signaling from the cell surface by binding to two different receptors (R: Type I and II). The heterodimeric formation of type I R and II R may occur before or after BMP binding, inducing signal transduction pathways through SMADs.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 2.2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 13.75 kDa.
  The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: His238-Leu347
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: NODAL nodal homolog (mouse) [ Homo sapiens (human) ]
• Official Symbol: NODAL
• Synonyms: NODAL; nodal homolog (mouse); nodal, mouse, homolog; nodal homolog; HTX5; MGC138230
• Gene ID: 4838
• mRNA Refseq: NM_018055
• Protein Refseq: NP_060525
• MIM: 601265
• UniProt ID: Q96S42