Active Recombinant Human NOTCH3 protein, Fc-tagged

• Cat.No.: NOTCH3-0545H
• Product Overview: Recombinant Human NOTCH3 protein(Ala40-Glu467), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 40-467 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human NOTCH3, Fc Tag at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (NOTCH2 × NOTCH3) with a linear range of 2-20 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 71.4 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala40-Glu467
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NOTCH3 notch 3 [ Homo sapiens ]
• Official Symbol: NOTCH3
• Synonyms: NOTCH3; notch 3; CADASIL, Notch (Drosophila) homolog 3 , Notch homolog 3 (Drosophila); neurogenic locus notch homolog protein 3; CASIL; Notch homolog 3; CADASIL
• Gene ID: 4854
• mRNA Refseq: NM_000435
• Protein Refseq: NP_000426
• MIM: 600276
• UniProt ID: Q9UM47