Active Recombinant Human NRG1 protein, mFc-tagged

• Cat.No.: NRG1-0537H
• Product Overview: Active Recombinant Human NRG1 protein(Ser 2-Lys 246)(Q02297-6) was expressed in HEK293, fused with the Fc region of mouse IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: 2-246 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its ability to bind with human ErbB4-His in a functional ELISA. 2. Measured by its ability to bind with human ErbB3-His in a functional ELISA.
• Molecular Mass: The recombinant human NRG1/mFc is a disulfide-linked homodimer. The reduced monomer comprises 481 amino acids and has a predicted molecular mass of 53.4 kDa. The apparent molecular mass of the monomer is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homo sapiens ]
• Official Symbol: NRG1
• Synonyms: NRG1; neuregulin 1; HGL; pro-neuregulin-1, membrane-bound isoform; GGF; HRG; NDF; MSTP131; pro-NRG1; glial growth factor; neu differentiation factor; neuregulin 1 type IV beta 3; neuregulin 1 type IV beta 1a; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); ARIA; GGF2; HRG1; HRGA; SMDF; MST131
• Gene ID: 3084
• mRNA Refseq: NM_001159995
• Protein Refseq: NP_001153467
• MIM: 142445
• UniProt ID: Q02297